Biostruct-X Workshop

Biostruct-X Workshop: Cryo Soft X-ray Imaging for Biological Applications

The first BioStruct- X Workshop on Cryo X-ray Imaging for Biological Applications will be held at the ALBA Synchrotron Light Source (Barcelona, Spain) on March 22nd 2013. The aim of the meeting is sharing ideas and experiences between experts and users with high, medium or low experience discussing on sample preparation, correlative microscopy and data processing.

http://www.biostruct-x.eu

Deadline: 15th of February 2013
Contact: biostruct@cells.es

Program

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Friday, March 22nd

Hard X-ray imaging

09:00

09:35

A. Somogyi (Soleil, France)
Hard X-ray nano-imaging/tomography at Nanoscopium (Soleil): a multi-technique tool for simultaneous trace metal and morphology studies of thick biological samples

Sample preparation and Correlative Microscopy

09:35

10:10

E. Duke (Diamond, UK)
How to increase the probability of getting good data: Take care over the sample preparation!

10:10

10:45

C. Hagen (Oxford U., UK)
Apoptosis observed in correlated fluorescence/Soft X-ray cryo-microscopy/tomography data

10:45

11:05

Coofee Break

11:05

11:40

J. Chichón (CNB-CSIC, Spain)
Imaging cells with soft X-ray microscopy

11:40

12:15

M. Elbaum (Weizmann Institute, Israel)
Dealing with depth: preparation and processing for thick specimens

12:15

13:00

Discussion - Chairman K. Grünewald

13:00

14:00

Lunch

Data processing for soft X-ray tomography

14:00

14:30

J.M. Carazo (CNB-CSIC, Spain)
Image formation and reconstruction in Soft X-ray microscopy with XMIPP

14:30

15:10

B. Heymann - Bsoft (Bethesda, USA)
Tools for electron and X-ray tomography in Bsoft

15:10

15:45

S. Marco - TomoJ (Marie Curie Inst., France)
Electron tomography and 3D chemical mapping based on ImageJ plugings TomoJ and EFTEM-TomoJ

15:45

16:20

D. Mastronarde - IMOD (Colorado, USA)
Tomographic reconstruction with the IMOD package

16:20

16:40

Coofee Break

16:50

17:35

Discussion - Chairman P. Guttmann

Future perspectives

17:35

18:10

P. Guttmann, G. Schneider (HZB, Germany)

Mistral TXM demonstration at the BL

18:10

19:00

Mistral TXM demonstration at the BL

END OF MEETING 19:00.

Getting to ALBA

Arriving by car

car ALBA-CELLS facilities are very close to the AP7 (connecting Barcelona with France) and C58 highways. From the Barcelona city center you can reach the site via C58 (Google Maps).

Arriving by plane

airport Barcelona International airport is the closest airport to ALBA-CELLS facilities. It is well connected to the Barcelona city center in approx. 30 minutes by train to downtown stations (BCN-Sants, BCN-Passeig de Gracia) and by bus to Catalunya Square (Plaça Catalunya).

Visit the AENA site for more information about the airport (or telephone +34 902 404 704).

You can get to ALBA by public transport by train and bus (please read below).

Arriving by train

train The Barcelona main train stations are BCN-Sants or BCN-Estació de França. Both stations are connected to the subway. Renfe is the Spanish railway company.

Getting to ALBA from Barcelona downtown in public transport

From Catalunya Square (Plaça Catalunya) you can use 2 train companies that offer you 2 options:

Take the line R4 of RENFE in direction to Terrassa/Manresa and stop at Cerdanyola del Vallès. Once there, two bus lines connect Cerdanyola del Vallès RENFE station with ALBA-CELLS site (Line A4 Cerdanyola del Vallès-Sant Cugat and Line B7 Cerdanyola del Vallès - Rubí). Bus stop is Sincrotrò.
Take the FGC (Ferrocarrils de la Generalitat de Catalunya) sign and take the lines , , or and stop at Sant Cugat station. Once you go out of the station by the Lluis Millet square exit, you have to take the bus ( Line A4 or Line B7). The bus stop is on the street "Carrer del Safareigs", when exiting the train station take the road fully on your right.